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Publications from the EM group at CSB, 1997 - 2003

Ellis, M. and Hebert, H. & Thelestam, M. (1997) Staphylococcus aureus alpha-toxin: Characterization of Protein/Lipid Interactions, 2D crystallization on Lipid Monolayers and 3D Structure, J. Struct. Biol., 118, 178-188.

Xian, Y. & Hebert, H. (1997) Three-Dimensional Structure of Porcine Gastric H,K-ATPase From Negatively Stained Crystals, J. Struct. Biol., 118, 169-177.

Hebert, H., Schmidt-Krey, I., Morgenstern, R., Murata, M., Hirai, T., Mitsuoka, K. & Fujiyoshi, Y. (1997) The 3.0 Å Projection Structure of Microsomal Glutathione Transferase as Determined by Electron Crystallography of p21212 Two-dimensional Crystals, J. Mol. Biol., 271, 751-758.

Ellis, M.J., Knapp, S., Koeck P.J.B., Fakoor-Biniaz, Z., Ladenstein, R. & Hebert, H. (1998) Two-dimensional Crystallization of the Molecular Chaperonin TF55 from the Hyperthermophilic Archaeon Sulfolobus solfataricus. J. Struct. Biol., 123, 30-36.

Schmidt-Krey, I., Lundqvist, G., Morgenstern, R. & Hebert, H. (1998) Parameters for the two-dimensional crystallization of the membrane protein microsomal glutathione transferase. J. Struct. Biol., 123, 87-96.

Koeck, P.J.B., Hebert, H., Ellis, M.J., Ladenstein, R. & Trent, J.D. (1998) Two-dimensional Crystals of Reconstituted Beta-subunits of the Chaperonin TF55 from Sulfolobus Shibatae. Biochim. Biophys. Acta, 1429, 40-44.

Hebert, H. (1998) Two-dimensional Crystals of Membrane Proteins. In Biomembrane Structures (Eds. D. Chapman and P. Haris), IOS Press, pp. 88-110.

Schmidt-Krey, I., Murata, K., Hirai, T., Morgenstern, R., Fujiyoshi, Y. & Hebert, H. (1999) The Projection Structure of the Membrane Protein Microsomal Glutathione Transferase at 3 Å Resolution as Determined from Hexagonal Two-dimensional Crystals, J. Mol. Biol., 288, 243-253.

Hesse, J., Hebert, H. and Koeck, P.J.B. (2000) Evaluation of Scanners and Comparison to CCD Cameras for High-Resolution TEM of Protein Crystals and Single Particles, Microsc. Res. Tech., 49, 292-300.

Schmidt-Krey, I., Mitsuoka, K., Hirai, T., Murata, K., Cheng, Y., Fujiyoshi, Y., Morgenstern, R. and Hebert, H.(2000) The three-dimensional map of microsomal glutathione transferase 1 at 6 Å resolution. EMBO J. 19, 6311-6316.

Hebert, H., Xian, Y., Thomsen, K. And Maunsbach, A.B. (2000) Structure of Renal Na,K-ATPase as observed by cryo-EM of 2-D crystals. In: Na/K-ATPase and Related ATPases, eds. Taniguchi, K. and Kaya, S., Elsevier Science, Amsterdam, pp 43-48.

P.J.B. Koeck (2000): “Ins and Outs of Digital Electron Microscopy”; Microscopy Research and Technique 49, 217-223.

P. J. B. Koeck (2000): “Quantization Errors in Averaged Digitized Data”; Signal Processing 81, 345-356.

Hebert, H., Purhonen, P., Vorum, H., Thomsen, K. and Maunsbach, A.B. (2001) Three-dimensional structure of renal Na,K-ATPase from cryo-electron microscopy of two-dimensional crystals. J. Mol. Biol. 314, 479-494.

Ellis, M.J. and Hebert, H. (2001) Structure analysis of soluble proteins using electron crystallography, Micron, 32, 541-550.

Ek. M., Engblom, D., Saha, S., Blomqvist, A., Jakobsson, P.J., Ericsson-Dahlstrand, A. (2001) Inflammatory response:Pathway across the blood-brain barrier. Nature, 410, 430-431.

Hacksell, I. Rigaud, J.-L., Purhonen, P., Pourcher, T., Hebert, H. and Leblanc, G. (2002) Projection structure at 8 Å resolution of the melibiose permease, an Na-sugar co-transporter from Escherichia coli, EMBO J., 21, 3569-3574.

Holm, P., Morgenstern, R. and Hebert, H. (2002) The 3-D structure of microsomal glutathione transferase 1 at 6 Å resolution as determined by electron crystallography of p22121 crystals. Biochim. Biophys. Acta, 1594, 276-285.

Engblom, D., Ek. M., Saha, S., Ericsson-Dahlstrand, A., Jakobsson, P.J., Blomqvist, A. (2002) Prostaglandins as inflammatory messengers across the blood-brain barrier. J. Mol. Med. 80, 5-15.

Hebert, H., Purhonen, P., Thomsen, K., Vorum, H. and Maunsbach, A.B. (2003) Renal Na,K-ATPase Structure from Cryo-electron Microscopy of Two-Dimensional Crystals. Ann N.Y. Acad. Sci. 986, 1-9.

Thorén, S., Weinander, R., Saha, S., Jegerschöld C., Pettersson P.L., Samuelsson, B., Hebert, H., Hamberg, M., Morgenstern, R., Jakobsson, P. J. (2003) Human Microsomal Prostaglandin E Synthase-1: Purification, Functional Characterization and Projection Structure Determination. J. Biol. Chem. In press.



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